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KMID : 0545120020120050807
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 5 p.807 ~ p.812
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A Novel Protein to Bind HCV Core Protein
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SO, KWAN YOUNG
LEE, HYANG JU/KANG, KWANG IL/LEE, HAYYOUNG/LIM, KYU/PAIK, SANG-GI/AHN, JEONG, KEUN
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Abstract
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In order to analyze the cellular proteins which interact with core protein of hepatitis C virus (HCV), a yeast two-hybrid screening technique was employed. A carboxyl terminus truncated core protein, which contained amino acid residues from the 1st to 120th, was used as a bait to screen cellular proteins. The expression library prepared from HeLa cell was screened and 400 positive clones were selected. The 75 clones from the positive clones were sequenced and analyzed by undergoing the Blast search. Interestingly, 7 out of the 75 clones encoded E7 antigen of human papilloma virus (HPV). We studied in detail the interaction between the truncated version of HCV core and E7 antigen in vitro. The core_120 protein expressed in chimeric form with GST was able to bring down the E7 protein of HPV type 18 expressed in bacteria. It is therefore suggested that the core of HCV might affect the interaction between E7 and a normal cellular tumor suppressor, known as Rb protein.
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KEYWORD
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